pcr reaction造句

例句与造句

1. After this initial construction phase, additional primers encompassing both ends are added to perform a regular PCR reaction, amplifying the target sequence away from all the shorter incomplete fragments.
2. Mitochondrial DNA analysis might be susceptible to contamination by nuclear mitochondrial DNA sequences co-amplified during the PCR reaction, thus confounding the analysis of homoplasmic versus heteroplasmic mitochondrial DNA.
3. A final concern is that bisulfite treatment greatly reduces the level of complexity in the sample, which can be problematic if multiple PCR reactions are to be performed ( 2006 ).
4. However, whenever I do any type of PCR reaction ( whether it's mutagenesis or straight up generating amplicons for subcloning ), I always make the master mix " without " the enzyme.
5. An extension of this approach includes an accurate model of the entire PCR reaction profile, which allows for the use of high signal-to-noise data and the ability to validate data quality prior to analysis.
6. It's difficult to find pcr reaction in a sentence. 用pcr reaction造句挺难的
7. One lab area is dedicated to preparation and handling of pre-PCR reagents and the setup of the PCR reaction, and another area to post-PCR processing, such as gel electrophoresis or PCR product purification.
8. The way that I was told do set up a PCR reaction was to insert the DNA samples into those strips of 0.2 ml tubes, and then add the'master-mix'containing everything else.
9. For the setup of PCR reactions, many standard operating procedures involve using pipettes with filter tips and wearing fresh laboratory gloves, and in some cases a laminar flow cabinet with UV lamp as a work station ( to destroy any extraneomultimer formation ).
10. :Real Time RT-PCR is becoming the standard for quantifying expression / mRNA levels-it's basically a way of normalizing each sample and allowing them to all be within the exponential phase of the PCR reaction with minimal operator intervention.
11. If I ran a PCR reaction and one of my primers was not working, for whatever reason, is there any possibility to have no bands appear under UV light after gel electrophoresis ? talk ) 17 : 21, 28 November 2008 ( UTC)
12. At present, mass diagnoses from a great number of samples cannot yet be achieved by HDA, whereas PCR reactions carried out in thermal cycler that can hold multi-well sample plates allows for the amplification and detection of the intended DNA target from a maximum of 96 samples.
13. After three rounds of contaminated PCR reactions, presumably a result of contamination of the pipette tip used to add the master-mix, I decided to avoid the possibility altogether by adding the master-mix to all empty tubes and then adding the samples to the master-mix.
14. In the second round of PCR, the complementary strand to the allele-specific forward primer is generated when the common reverse primer binds to the amplicon formed in the first round of PCR . Finally, the thermocycling of the PCR reaction continues, starting the third portion of the KASP method.
15. Because most eukaryotic genes contain introns, which are present in the genome but not in the mature mRNA, the cDNA generated from a RT-PCR reaction is the exact ( without regard to the error-prone nature of reverse transcriptases ) DNA sequence that would be directly translated into protein after transcription.
16. As a result, if a given allele is present in the PCR reaction, the primer pair specific to that allele will produce product but not to the alternative allele with a different SNP . The two primer pairs are also designed such that their PCR products are of a significantly different length allowing for easily distinguishable bands by gel electrophoresis or melt temperature analysis.
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